Metabolism of branched-chain amino acids in starved rats: the role of hepatic tissue

Title:

Metabolism of branched-chain amino acids in starved rats: the role of hepatic tissue

Creator:

Milan Holeček, Šprongl, L., and Ivan Tilšer

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:142651c1-4ab4-4d1e-97df-2366cb827105
uuid:142651c1-4ab4-4d1e-97df-2366cb827105
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, racionální výživa, hladovění, metabolismus bílkovin, nutrition, starvation, protein metabolism, leucine, branched-chain amino acids, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Parameters of branched-chain amino acids (BCAA; leucine, isoleucine and valine) and protein metabolism were evaluated using L-[1-14C]leucine and a-keto[1-14C]isocaproate (KIC) in the whole body and in isolated perfused liver (IPL) of rats fed ad libitum or starved for 3 days. Starvation caused a significant increase in plasma BCAA levels and a decrease in leucine appearance from proteolysis, leucine incorporation into body proteins, leucine oxidation, leucine-oxidized fraction, and leucine clearance. Protein synthesis decreased significantly in skeletal muscle and the liver. There were no significant differences in leucine and KIC oxidation by IPL. In starved animals, a significant increase in net release of BCAA and tyrosine by IPL was observed, while the effect on other amino acids was non-significant. We conclude that the protein-sparing phase of uncomplicated starvation is associated with decreased whole-body proteolysis, protein synthesis, branched-chain amino acid (BCAA) oxidation, and BCAA clearance. The increase in plasma BCAA levels in starved animals results in part from decreased BCAA catabolism, particularly in heart and skeletal muscles, and from a net release of BCAA by the hepatic tissue., M. Holeček, L. Šprongl, I. Tilšer., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2001 Volume:50 | Number:1

Harvested from:

CDK

Metadata only:

false