Aquaporin expression in the fetal porcine urinary tract changes during gestation

Title:

Aquaporin expression in the fetal porcine urinary tract changes during gestation

Creator:

Jakobsen, L. K, Trelborg, K. F, Olsen, L. H, Nørregaard, R, Djurhuus, J. C, Andersson, K.-E, Høyer, S, and Kingo, P. S

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:143f4c9a-a033-4be1-877b-1fc017b018fc
uuid:143f4c9a-a033-4be1-877b-1fc017b018fc
issn:0862-8408

Subject:

fyziologie člověka, human physiology, Aquaporins, Urinary tract, Fetal development, Pig, Protein expression, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

The expression of aquaporins (AQPs ) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11 ), QPCR (AQPs positive on RT-PCR), and immunohistochemistry. Bladder samples were additionally examined by Western blotting. RNA was extracted from 76 tissue samples obtained from 19 fetuses. Gestational age was 60 (n=11) or 100 days (n=8). PCR showed that AQP1, 3, 9 and 11 mRNA was expressed in all locations. The expression of AQP3 increased significantly at all four locations with gestational age, whereas AQP11 significantly decreased. AQP1 expression increased in the ureter, bladder and urethra. AQP9 mRNA expression increased in the urethra and bladder, but decreased in the ureter. AQP5 was expressed only in the urethra. Immunohistochemistry showed AQP1 staining in sub-urothelial vessels at all locations. Western blotting analysis confirmed increased AQP1 protein levels in bladder samples during gestation. Expression levels of AQP1, 3, 5, 9 and 11 in the urinary tract change during gestation, and further studies are needed to provide insights into normal and pathophysiological water handling mechanisms in the fetus., L. K. Jakobsen, K. F. Trelborg, P. S. Kingo, S. Høyer, K.-E. Andersson, J. C. Djurhuus, R. Nørregaard, L. H. Olsen., and Seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:2

Harvested from:

CDK

Metadata only:

false