Differential expression of VEGFA, TIE2, and ANG2 but not ADAMTS1 in rat mesenteric microvascular arteries and veins

Title:

Differential expression of VEGFA, TIE2, and ANG2 but not ADAMTS1 in rat mesenteric microvascular arteries and veins

Creator:

Mecha Disassa, N., Styp-Rekowska, B., Hinz, B., Da Silva-Azevedo, L., Pries, A. R., and Zakrzewicz, Andreas

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:15837865-7eb2-415a-a862-bb7cf2cd5327
uuid:15837865-7eb2-415a-a862-bb7cf2cd5327

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, endotel, růstové faktory, physiology, endothelium, growth factors, microcirculation, vascular endothelial growth factor A, receptor TIE2, angiopoietin-2, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Microvessels respond to metabolic stimuli (e.g. pO2) and hemodynamic forces (e.g. shear stress and wall stress) with structural adaptations including angiogenesis, remodeling and pruning. These responses could be mediated by differential gene expression in endothelial and smooth muscle cells. Therefore, rat mesenteric arteries and veins we reexcised by microsurgery, and mRNA expression of four angioadaptation-related genes was quantified by real time duplex RT-PCR in equal amounts of total RNA, correlated to two different house keeping genes (ß-actin, GAPDH). The results show higher expression of VEGFA, TIE2, and ANG2 in arteries than in veins, but equal expression of ADAMTS1. Higher availability of VEGFA mRNA in endothelial cells of arteries shown here could contribute to the maintenance of mechanically stressed blood vessels and counteract pressure-induced vasoconstriction., N. Mecha Disassa ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2009 Volume:58 | Number:2

Harvested from:

CDK

Metadata only:

false