A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection

Title:

A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection

Creator:

Choi, Yong Soo, Lee, Kwang Sik, Yoon, Hyung Joo, Kim , Iksoo, Sohn, Hung Dae, and Jin, Byung Rae

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1b723216-cbc4-42a8-8512-214f47b3f3d0
uuid:1b723216-cbc4-42a8-8512-214f47b3f3d0

Subject:

Antioxidant enzyme, Apidae, Bombus ignitus, gene structure, oxidative stress, reactive oxygen species (ROS), thioredoxin-like protein (txl), and bumblebee

Type:

article, model:article, and TEXT

Description:

A thioredoxin-like protein (txl) gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus txl (Bitxl) gene spans 1777 bp and consists of three introns and four exons coding for 285 amino acid residues with a conserved active site (CGPC). The deduced amino acid sequence of the Bitxl cDNA was 65% similar to the Drosophila melanogaster txl. Northern blot analysis revealed the presence of Bitxl transcripts in all tissues examined. When H2O2 was injected into the body cavity of B. ignitus workers, Bitxl mRNA expression was up-regulated in the fat body tissue. In addition, the expression levels of Bitxl mRNA in the fat body greatly increased when B. ignitus workers were exposed to low (4°C) or high (37°C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that the Bitxl possibly protects against oxidative stress caused by extreme temperatures and bacterial infection.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2006 Volume:103 | Number:2

Harvested from:

CDK

Metadata only:

false