Nucleotide diversity based on csd gene of the black giant honey bee, Apis laboriosa (Hymenoptera: Apidae)

Title:

Nucleotide diversity based on csd gene of the black giant honey bee, Apis laboriosa (Hymenoptera: Apidae)

Creator:

Wang, Huan, Wang, Zi-Long, Zeng, Zhi-Jiang, Wu, Xiao-Bo, and Yan, Wei-Yu

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1c79faca-f05c-4fcb-9420-2378166734f3
uuid:1c79faca-f05c-4fcb-9420-2378166734f3

Subject:

Zoologie, zoologie, entomologie, Hymonoptera, Apidae, Apis laboriosa, complementary sex determination, csd haplotype, nucleotide diversity, 2, and 59

Type:

article, články, model:article, and TEXT

Description:

The mechanism of sex determination is common for all honeybee species (Apis spp.) by the complementary sex determination (csd) gene. The csd gene has been studied in the Western honey bee (Apis mellifera L.), the Eastern honey bee (Apis cerana F.) and the giant honey bee (Apis dorsata F.), whereas no studies had been conducted on the high altitude Himalayan or black giant honey bee, Apis laboriosa Smith. In the present study, we cloned the genomic exon 6 to exon 9 region of the A. laboriosa csd gene, and identified 13 csd haplotypes. The data was analyzed and compared with the other aforementioned three honeybee species. The results showed that, as with the other three Apis species, region 3 of the csd gene contains an RS domain at the N terminal, a proline-rich domain at the C terminal, and a hypervariable region in the middle. A phylogenetic tree showed that the csd haplotypes from A. laboriosa fell into one clade with those from A. dorsata, and were separated from those from the other two species, A. mellifera and A. cerana. The network map also showed that the csd haplotypes from A. laboriosa and A. dorsata are well mixed among each other, and do not form two separate branches. Pairwise Fst analysis revealed that the value between A. laboriosa and A. dorsata was very low (0.098), confirming a close relationship to exist between them., Huan Wang ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2013 Volume:110 | Number:2

Harvested from:

CDK

Metadata only:

false