Optimal wire myography normalization for the rat dorsal penile, internal pudendal and internal iliac arteries

Title:

Optimal wire myography normalization for the rat dorsal penile, internal pudendal and internal iliac arteries

Creator:

Azeez, Tooyib A., Andrade, Manuella R., and La Favor, Justin D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1edf11dc-96a1-426e-b14f-5b5e4b667a30
uuid:1edf11dc-96a1-426e-b14f-5b5e4b667a30
doi:10.33549/physiolres.934714

Subject:

normalization procedure, optimal initial tension, myograph, pre-penile arteries, and erectile dysfunction

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

In functional arterial studies using wire myography, the determination of a vessel’s standardized normalization factor (factor k) is an essential step to ensure optimal contraction and relaxation by the arteries when stimulated with their respective vasoactive agents and to obtain reproducible results. The optimal factor k for several arteries have been determined, however, the optimal initial tension and factor k for the arteries involved in erection remains unknown. Hence, in the present study we set out to determine the optimal factor k for the internal iliac artery, proximal and distal internal pudendal artery (IPA), and dorsal penile artery. After isolating, harvesting, and mounting the arteries from male Sprague-Dawley rats on a multi wire myograph, we tested arterial responsivity to high K+-stimulation when the factor k was set at 0.7, 0.8, 0.85, 0.9, 0.95, 1.0, 1.1, and 1.2 to determine the factor k setting that results in the greatest K+-induced active force production for each vessel type. The data showed the optimal factor k is 0.90-0.95 for the dorsal penile, distal internal pudendal and internal iliac arteries while it is 0.85-0.90 for proximal internal pudendal artery. These optimal values corresponded to initial passive tension settings of 1.10±0.16 - 1.46±0.23, 1.28±0.20 - 1.69±0.34, 1.03±0.27 - 1.33±0.31, and 1.33±0.31 - 1.77±0.43 mN/mm for the dorsal penile, distal IP, proximal IP, and internal iliac arteries, respectively.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

931-937

Source:

Physiological research | 2021 Volume:70 | Number:6

Harvested from:

CDK

Metadata only:

false