Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men

Title:

Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men

Creator:

Vigelsø, A., Prats, C., Ploug, T., Dela, F., and Helge, J. W.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:20fbd0e5-0710-40b9-8152-8543f264b64a
uuid:20fbd0e5-0710-40b9-8152-8543f264b64a
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, metabolismus lipidů, vytrvalostní trénink, muži středního věku, lipid metabolism, endurance training, middle aged men, glucose metabolism, human skeletal muscle, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

A high VO2max in middle-age is related to high metabolic flexibility and lowered risk of metabolic diseases. However, the influence of a high VO2max induced by years of regular training in middle-age on protein expression related to muscle metabolism is not well studied. This study measures key proteins involved in mitochondrial oxidation, glucose and lipid metabolism in skeletal muscle of trained and untrained middle-aged men. 16 middle-aged men, matched for lean body mass, were recruited into an endurance trained (TR, n=8) or an untrained (CON, n=8) group based on their VO2max. A muscle biopsy was obtained from m. vastus lateralis and protein levels were analyzed by Western blotting. The TR had higher protein levels of mitochondrial complex III-V, endothelial lipase (EL) and perilipin 5 compared to the CON. Glycogen synthase (P=0.05), perilipin 3 (P=0.09) and ATGL (P=0.09) tended to be higher in TR than CON, but there was no difference in AKT I/II, HKII, GLUT4 and LPL protein expression. Lastly, there was a positive correlation between plasma HDL and EL (R2=0.53, P<0.01). In conclusion, a high VO2max in middle-aged men was as expected is reflected in higher muscle oxidative capacity, but also in higher endothelial lipase and perilipin 5 expression and a borderline higher glycogen synthase protein expression, which may contribute to a higher metabolic flexibility., A. Vigelsø, C. Prats, T. Ploug, F. Dela, J. W. Helge., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:2

Harvested from:

CDK

Metadata only:

false