Effect of hindlimb unweighting on expression of hypoxia-inducible factor-1α, vascular endothelial growth factor, angiopoietin, and their receptors in mouse skeletal muscle

Title:

Effect of hindlimb unweighting on expression of hypoxia-inducible factor-1α, vascular endothelial growth factor, angiopoietin, and their receptors in mouse skeletal muscle

Creator:

Wagatsuma, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:22e9ba74-b97a-49a3-99dd-173c8c993379
uuid:22e9ba74-b97a-49a3-99dd-173c8c993379
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, fyziologie, končetiny, svalová atrofie, physiology, extremities, muscular atrophy, anigiogenic factor, capillary regression, hindlimb unweighting, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Hindlimb unweighting (HU) leads to capillary regression in skeletal muscle. However, the molecular mechanism(s) remains to be elucidated. To gain insight into the regulation of this process, we investigated gene expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), angiopoietin, and their receptors in the atrophied muscle induced by HU. The hindlimbs of mice were unweighted by tail-suspension and then the gastrocnemius muscles were isolated after 10 days. To assess the capillary distribution, the capillary endothelium in frozen transverse sections was identified by staining for alkaline phosphatase. The mRNA levels were analyzed using a real-time reverse transcription-polymerase chain reaction. After 10 days of HU, the number of capillaries around a muscle fiber was significantly decreased by 19.5 %, suggesting that capillary regression appears to occur. The expression of HIF-1α was significantly down-regulated after 10 days of HU. The expression of VEGF remained unchanged, whereas those of Flt-1, KDR/Flk-1, and neuropilin-1 were significantly down-regulated, suggesting that VEGF signaling through these receptors would be attenuated. The expression of angiopoietin-1, and -2, as well as their receptor, Tie-2 were also significantly down-regulated, suggesting that angiopoietin-1 signaling through Tie-2 would be attenuated. These findings suggest that alterations in expression of VEGF, angiopoietins, and their receptors may be associated with capillary regression after HU., A. Wagatsuma., and Obsahuje biblografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:4

Harvested from:

CDK

Metadata only:

false