Application of proteomics in biomarker discovery: a primer for the clinician

Title:

Application of proteomics in biomarker discovery: a primer for the clinician

Creator:

Tambor, V., Alena Fučíková, Juraj Lenčo, Marian Kacerovský, Vít Řeháček, Jiří Stulík, and Radek Pudil

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:239f68c3-d536-4cfb-8dc7-6d8ad52ebc23
uuid:239f68c3-d536-4cfb-8dc7-6d8ad52ebc23

Subject:

Fyziologie člověka a srovnávací fyziologie, proteomika, hmotnostní spektrometrie, proteomics, mass spectroscopy, biomarker, plasma, mass spectrometry, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Ever since proteomics was proven to be capable of characterizing a large number of differences in both protein quality and quantity, it has been applied in various areas of biomedicine, ranging from the deciphering molecular pathogenesis of diseases to the characterization of novel drug targets and the discovery of potential diagnostic biomarkers. Indeed, the biomarker discovery in human plasma is clearly one of the areas with enormous potential. However, without proper planning and implementation of specific techniques, the efforts and expectations may very easily be hampered. Numerous earlier projects aimed at clinical proteomics, characterized by exaggerated enthusiasm, often underestimated some principal obstacles of plasma biomarker discovery. Consequently, ambiguous and insignificant results soon led to a more critical view in this field. In this article, we critically review the current state of proteomic approaches for biomarker discovery and validation, in order to provide basic information and guidelines for both clinicians and researchers. These need to be closely considered prior to initiation of a project aimed at plasma biomarker discovery. We also present a short overview of recent applications of clinical proteomics in biomarker discovery., V. Tambor ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:4

Harvested from:

CDK

Metadata only:

false