Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Title:

Pro-inflammatory gene expression in adipose tissue of patients with atherosclerosis

Creator:

Soňa Čejková, Králová Lesná, I., Jiří Froněk, Libor Janoušek, Králová, A., Jana Ždychová, and Rudolf Poledne

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:23c47fc8-67a6-460c-adfb-9755435cc01e
uuid:23c47fc8-67a6-460c-adfb-9755435cc01e
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, ateroskleróza, tuková tkáň, genová exprese, atherosclerosis, adipose tissue, gene expression, 14, and 612

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNFα), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFα 5-fold, p<0.05; MCP-1 3.6-fold, p<0.05; IL-6 18.8-fold, p<0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFα concentration in patients (4.86± 1.4 pg/ml) compared to LKDs (2.14±0.9 pg/ml; p<0.001), as was hsCRP (11.8±7.0 in PAD; 1.5±0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFα and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis., S. Čejková, I. Králová Lesná, J. Froněk, L. Janoušek, A. Králová, J. Ždychová, R. Poledne., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:4

Harvested from:

CDK

Metadata only:

false