The dynamics of gene expression in human lung microvascular endothelial cells after stimulation with inflammatory cytokines

Title:

The dynamics of gene expression in human lung microvascular endothelial cells after stimulation with inflammatory cytokines

Creator:

Zachlederová, M. and Jarolím, P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:26610dd5-f3c2-4787-afb8-aa6bef483c11
uuid:26610dd5-f3c2-4787-afb8-aa6bef483c11

Subject:

Vasoocclusion, TNF α, IL-1 β, Microvascular endothelial cells, Gene expression, and cDNA array

Type:

article, model:article, and TEXT

Description:

Vascular endothelium plays an essential role in the pathogenesis of vasoocclusion. The changes in the endothelial cell function can be triggered by changes in gene expression caused by interaction with cytokines and blood cells. Using cDNA arrays, we have recently reported complex patterns of gene expression after stimulation of endothelial cells with TNFα and IL-1β. Better understanding of the time course of gene expression changes, their concentration dependence and reversibility after withdrawal of the offending cytokine is essential for successful prevention and therapy of vasoocclusion. Here we present a detailed study of the concentration dependence and time course of gene expression in endothelial cells after their exposure to TNFα and IL-1β. We focus on the adhesion molecules (VCAM-1, ICAM-1, E-selectin) and cytokines (IL-6, GCP-2, MCP-1) that are likely to contribute to vasoocclusion. We report differences in the time course and intensity of their expression and in their response to TNFα and IL-1β stimulation. We demonstrate that expression of the studied genes is upregulated by low TNFα concentrations that better reflect the TNFα levels detected in the plasma of patients developing vasoocclusion. These results help to understand the changes in the endothelium and to design rational prevention and therapy of vasoocclusion.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:1

Harvested from:

CDK

Metadata only:

false