Fluid shear stress promotes osteoblast proliferation and suppresses mitochondrial-mediated osteoblast apoptosis through the miR-214-3p-ATF4 signaling axis

Title:

Fluid shear stress promotes osteoblast proliferation and suppresses mitochondrial-mediated osteoblast apoptosis through the miR-214-3p-ATF4 signaling axis

Creator:

Zhang, Kun, Liu, Xuening, Tang, Yuchen, Liu, Zhongcheng, Yi, Qiong, Wang, Lifu, Geng, Bin, and Xia, Yayi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:27421fea-6dbb-4a52-bd83-28016f52f1a2
uuid:27421fea-6dbb-4a52-bd83-28016f52f1a2
doi:10.33549/physiolres.934917

Subject:

fluid shear stress, miR-214-3p, activating transcription factor 4, osteoblast, proliferation, and apoptosis

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

MicroRNAs (miRNAs) play vital roles in bone metabolism and participate in the mechanically induced bone alterations. The underlying molecular mechanisms by which fluid shear stress (FSS) regulate the proliferative and apoptotic phenotypic changes of osteoblasts remain elusive. The study aimed to investigate the regulatory effects of FSS on osteoblast proliferative and apoptotic phenotypes and the roles of miR-214-3p-ATF4 (activating transcription factor 4) signaling axis in the mechanomodulation processes. FSS promoted the proliferative activity of osteoblasts and suppressed mitochondrial-mediated osteoblast apoptosis. FSS decreased miR-214-3p expression and increased ATF4 expression in MC3T3-E1 osteoblasts. MiR-214-3p inhibited osteoblast proliferative activity and promoted mitochondrialmediated osteoblast apoptosis. Overexpression of miR-214-3p attenuated FSS-enhanced osteoblast proliferation and FSS-suppressed mitochondrial-mediated osteoblast apoptosis. We validated that ATF4 acted as a target gene of miR-214-3p. Moreover, miR-214-3p regulated osteoblast proliferation and apoptosis through targeting ATF4. Taken together, our study proved that FSS could suppress mitochondrial-mediated osteoblast apoptosis and promote osteoblast proliferation through the miR-214-3p-ATF4 signaling axis.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

527-538

Source:

Physiological research | 2022 Volume:71 | Number:4

Harvested from:

CDK

Metadata only:

false