Coupling cofactor - a novel regulatory component in G protein-mediated signalling?

Title:

Coupling cofactor - a novel regulatory component in G protein-mediated signalling?

Creator:

Nanoff, C. and Freissmuth, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2b0a60d9-0ae6-491a-b286-681b4a073b57
uuid:2b0a60d9-0ae6-491a-b286-681b4a073b57

Subject:

G proteins, adenosine receptors, dopamine receptors, guanine nucleotides, and signal transduction

Type:

article, model:article, and TEXT

Description:

The model for studying the mechanism of G protein-mediated signalling cannot account for the observation that high-affinity binding of agonists to many different receptors is not dissociated by the addition of high concentrations of guanine nucleotides. Using the cerebral Ai-adenosine receptor as a model system, we have recently identified a component which is responsible for this phenomenon. This protein, termed the coupling cofactor, can be solubilized from brain membranes and chromatographically resolved from both the G proteins and the receptor. Following reconstitution into appropriate acceptor membranes, the coupling cofactor confers resistance of high-affinity agonist binding to guanine nucleotides. The coupling cofactor acts as a brake and limits receptor-dependent signal amplification; in addition, it is a candidate for participating in the higher level organization of receptors and G proteins in membranes and in the membrane-delimited cross-talk between individual receptors. Here, we present a working hypothesis on the possible biological roles of the coupling cofactor.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1997 Volume:46 | Number:2

Harvested from:

CDK

Metadata only:

false