A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Title:

A gypsy moth (Lymantria dispar, Lepidoptera: Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain

Creator:

Narang, Neelam, Hérard, Franck, Dougherty, Edward M., Chen, Kim, and Vega, Fernardo E.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2d28e9dc-5335-468b-9015-0d4cdf5ba8f6
uuid:2d28e9dc-5335-468b-9015-0d4cdf5ba8f6

Subject:

Biocontrol, microbial control, insect pathogens, baculovirus, NPV, nucleopolyhedrovirus, gypsy moth, Lymantria dispar, and fluorescent brightener

Type:

article, model:article, and TEXT

Description:

As part of a search for natural enemies of the gypsy moth (Lymantria dispar), virus-infected samples were collected near Toulouse, France. Light and electron microscope studies confirmed that the French strain is a multinucleocapsid nuclear polyhedrosis virus (MNPV). In vivo bioassays using the New Jersey strain of L. dispar, and comparing L. dispar MNPV (LdMNPV) strains from France, North America and Korea, showed that the French strain was the least active, whereas the North American strain had the highest activity. The viral efficacy of all strains was enhanced 200 to 1300-fold in the presence of 1% fluorescent brightener. The enhancement was highest in the American strain and lowest in the French strain. French LdMNPV (LdMNPVF) DNA cut with four restriction enzymes (BamHI, EcoRI, HindIII, and NotI) revealed minor fragment size differences, but many similarities when compared to the North American and the Korean strain. PCR amplification of a LdMNPV early gene (G22) was detected in the North American and the Korean strain, but not in the French strain.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2001 Volume:98 | Number:2

Harvested from:

CDK

Metadata only:

false