Response of human HTB140 melanoma cells to conventional radiation and hadrons

Title:

Response of human HTB140 melanoma cells to conventional radiation and hadrons

Creator:

Ristić-Fira, A., Todorović, D., Žakula, J., Keta, O., Cirrone, P., Cuttone, G., and Petrović, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3077ac24-53d5-4a92-9e14-6d6ba36bd05b
uuid:3077ac24-53d5-4a92-9e14-6d6ba36bd05b

Subject:

Fyziologie člověka a srovnávací fyziologie, protony, přežití, protons, survival, carbon ions, HTB140 melanoma, proliferation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Conventional radiotherapy with X- and γ-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and 12C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with γ-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u 12 C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than γ-rays. The best efficiency was obtained with 12C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for 12C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and 12C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance., A. Ristić-Fira ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false