Real time RT-PCR with a newly designed set of primers confirmed the presence of 2b and 2x/d myosin heavy chain mRNAs in the rat slow soleus muscle

Title:

Real time RT-PCR with a newly designed set of primers confirmed the presence of 2b and 2x/d myosin heavy chain mRNAs in the rat slow soleus muscle

Creator:

Jitka Žurmanová, František Půta, Stopková, R., and Tomáš Soukup

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3179fc7c-7a47-4cbc-9e4d-e2ad933b732b
uuid:3179fc7c-7a47-4cbc-9e4d-e2ad933b732b
issn:0862-8408

Subject:

Experimentální medicína, fyziologie, potkan, svaly dolních končetin, physiology, Rattus norvegicus, lower limb muscles, rat slow soleus muscle, rat fast EDL muscle, myosin heavy chain isoforms, real time RT-PCR, 14, and 616-092

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In order to re-evaluate the presence and relative quantity of 2b and 2x/d myosin heavy chain (MyHC) transcripts in rat slow soleus muscle by using real time RT-PCR we have compared the available relevant cDNA sequences and designed a new set of primers having similar melting temperatures, matching separate MyHC exons in the regions of maximal differences in MyHC coding sequences, and containing G or C at the 3́-end. These also yielded PCR products of corresponding length, which is an important requirement for real time RT-PCR quantification. The experiments were performed on 8- month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected, J. Žurmanová, F. Půta, R. Stopková, T. Soukup., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:6

Harvested from:

CDK

Metadata only:

false