Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models

Title:

Liver glucocorticoid receptor and heat shock protein 70 levels in rats exposed to different stress models

Creator:

Filipović, D., Gavrilović, L., Dronjak, S., Demajo, M., and Marija B. Radojčić

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:31c9d163-6fdd-4580-8871-f03fd40e8b19
uuid:31c9d163-6fdd-4580-8871-f03fd40e8b19
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, molekulární biologie, potkan, játra, stres (fyziologie), glukokortikoidy, molecular biology, Rattus norvegicus, liver, stress (physiology), glucocorticoids, protein 70 teplotního šoku, heat shock protein 70, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The aim of the present study was to define the stress-induced pattern of cytosolic glucocorticoid receptor (GR) and Hsp70 protein in the liver of male Wistar rats exposed to different stress models: acute (2 h/day) immobilization or cold (4 °C); chronic (21 days) isolation, crowding, swimming or isolation plus swimming and combined (chronic plus acute stress). Changes in plasma levels of corticosterone were studied by radioimmunoassay (RIA). The results obtained by Western immunoblotting showed that both acute stressors led to a significant decrease in cytosolic GR and Hsp70 levels. Compared to acute stress effects, only a weak decrease in the levels of GR and Hsp70 was demonstrated in chronic stress models. Chronically stressed rats, which were subsequently exposed to novel acute stressors (immobilization or cold), showed a lower extent of GR down-regulation when compared to acute stress. The exception was swimming, which partially restores this down-regulation. The observed changes in the levels of these major stress-related cellular proteins in liver cytosol lead to the conclusion that chronic stressors compromise intracellular GR down-regulation in the liver., D. Filipović, L. Gavrilović, S. Dronjak, M. Demajo, M. B. Radojčić., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:2

Harvested from:

CDK

Metadata only:

false