Effect of agroclavine on NK activity in vivo under normal and stress conditions in rats

Title:

Effect of agroclavine on NK activity in vivo under normal and stress conditions in rats

Creator:

Miroslav Starec, Anna Fišerová, Jozef Rosina, Jiří Málek, and Miloslav Kršiak

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:379d3977-d1bf-491f-823a-98549de92a07
uuid:379d3977-d1bf-491f-823a-98549de92a07
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, stres (fyziologie), toxicita, stress (physiology), toxicity, agroclavine, ergot alkaloid, rat, NK cell activity, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Agroclavine is a natural, clavine type of ergot alkaloid with D1 dopamine and ?-adrenoceptor agonistic properties. We showed previously that in vitro agroclavine enhances natural killer (NK) cell activity, increases interleukin-2 and interferon-gamma production and prolongs the survival time of tumor-bearing mice. The aim of this study was 1) to test the effect of agroclavine on NK activity in vivo, and 2) to assess the potential toxicity of high doses of agroclavine on cardiac and liver functions using creatine kinase MB (CKMB) and alanine aminotransferase (ALT) as biochemical markers in normal and stressed animals. The effect of stress was studied because we examined promising anticancer properties of agroclavine and malignant diseases are supposed to be a potent stressful event for patients. In our experiments 3-month-old male rats of the Wistar-Kyoto strain were used. Agroclavine was injected intraperitoneally (0.5 mg/kg or 0.05 mg/kg) 30 min before stress (four hours' restraint and immersion in 23 °C water). The animals were killed 30 min after stress, blood was collected and the spleen was removed. Non-stressed animals treated with agroclavine were killed 5 h after the drug administration. The results confirmed our previous in vitro results and showed that also in vivo agroclavine increases NK cell activity under non-stress conditions. Agroclavine only slightly increased CKMB and had no influence on ALT in non-stressed animals. These promising results are limited by the fact that agroclavine (0.5 mg/kg) diminished NK cell activity and significantly increased ALT and CKMB under stress conditions., M. Starec, A. Fišerová, J. Rosina, J. Málek, M. Kršiak., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2001 Volume:50 | Number:5

Harvested from:

CDK

Metadata only:

false