Effect of fast protein liquid chromatography fractionated salivary gland extracts from different ixodid tick species on interleukin-8 binding to its cell receptors

Title:

Effect of fast protein liquid chromatography fractionated salivary gland extracts from different ixodid tick species on interleukin-8 binding to its cell receptors

Creator:

Kocáková, Paulína, Sláviková, Monika, Hajnická, Valéria, Slovák, Mirko, Gašperík, Juraj, Vančová, Iveta, Fuchsberger, Norbert, and Nuttall, Patricia A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3823e849-d74d-46a6-aad1-8c4459131724
uuid:3823e849-d74d-46a6-aad1-8c4459131724
doi:10.14411/fp.2003.014

Subject:

Dermacentor reticulatus, Rhipicephalus appendiculatus, Amblyomma variegatum, interleukin-8, tick saliva, chemokine inhibitors tick species - Dermacentor reticulatus (Fabricius, 1794), Rhipicephalus appendiculatus Neumann, 1901, and and Amblyomma

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Interleukin-8 plays a critical role in inflammatory processes. Hence generation of molecules with anti-IL-8 activity is likely to be important for successful feeding and for survival of the ticks. Anti-IL-8 activity was studied in saliva of three ixodid tick species - Dermacentor reticulatus (Fabricius, 1794), Rhipicephalus appendiculatus Neumann, 1901, and Amblyomma variegatum (Fabricius, 1794). The greatest activity was shown in saliva prepared from D. reticulatus. The activity was attributed to tick salivary gland molecules that bind to IL-8, preventing binding of the chemokine to its specific receptor, rather than to occupation of the IL-8 cell receptor by the tick molecules. The distribution of anti-IL-8 activity in fast protein liquid chromatography (FPLC) fractions of salivary gland extracts (SGE) derived from adult female D. reticulatus, R. appendiculatus and A. variegatum was compared directly by both ELISA and receptor-binding inhibition assays. The correspondence in results with fractions of SGE from ELISA is consistent with detection of tick molecules that inhibit IL-8 binding to its receptor. As IL-8 is an important chemoattractant and activator of neutrophils, the presence of an anti-IL-8 activity in tick saliva indicates that neutrophils play an important role in the host response to parasitism by ticks.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

79-84

Source:

Folia parasitologica | 2003 Volume:50 | Number:1

Harvested from:

CDK

Metadata only:

false