Hypothermic preservation of rat hearts using antifreeze glycoprotein

Title:

Hypothermic preservation of rat hearts using antifreeze glycoprotein

Creator:

Takago, Shintaro, Matsumoto, Isao, Kato, Hiroki, Saito, Naoki, Ueda, Hideyasu, Iino, Kenji, Kimura, Keiichi, and Takemura, Hirofumi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3b1530d7-201f-4d13-80a9-5f27e5accc42
uuid:3b1530d7-201f-4d13-80a9-5f27e5accc42
doi:10.33549/physiolres.934473

Subject:

antifreeze glycoprotein, hypothermic preservation, and rat

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Antifreeze proteins are an effective additive for low-temperature preservation of solid organs. Here, we compared static hypothermic preservation with and without antifreeze glycoprotein (AFGP), followed by nonfreezing cryopreservation of rat hearts. The heart was surgically extracted and immersed in one of the cardioplegia solutions after cardiac arrest. Control rat hearts (n=6) were immersed in University of Wisconsin (UW) solution whereas AFGP-treated hearts (AFGP group) (n=6) were immersed in UW solution containing 500 μg/ml AFGP. After static hypothermic preservation, a Langendorff apparatus was used to reperfuse the coronary arteries with oxygenated Krebs-Henseleit solution. After 30, 60, 90, and 120 min, the heart rate (HR), coronary flow (CF), cardiac contractile force (max dP/dt), and cardiac diastolic force (min dP/dt) were measured. Tissue water content (TWC) and tissue adenosine triphosphate (ATP) levels in the reperfused preserved hearts were also assessed. All the parameters were compared between the control and AFGP groups. Compared with the control group, the AFGP group had significantly (p<0.05) higher values of the following parameters: HR at 60, 90, and 120 min; CF at all four time points; max dP/dt at 90 min; min dP/dt at 90 and 120 min; and tissue ATP levels at 120 min. TWC did not differ significantly between the groups. The higher HR, CF, max dP/dt, min dP/dt, and tissue ATP levels in the AFGP compared with those in control hearts suggested that AFGP conferred superior hemodynamic and metabolic functions. Thus, AFGP might be a useful additive for the static/nonfreezing hypothermic preservation of hearts.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1029-1038

Source:

Physiological research | 2020 Volume:69 | Number:6

Harvested from:

CDK

Metadata only:

false