JAB1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by regulating angiopoietin-like protein 2

Title:

JAB1 promotes high glucose-induced inflammation and extracellular matrix deposition in glomerular mesangial cells by regulating angiopoietin-like protein 2

Creator:

Zhu, P., Wu, Yihua, Gu, Y., and Li, C.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3d2e1d75-2422-4dc7-aaa5-37bc3fd3e05d
uuid:3d2e1d75-2422-4dc7-aaa5-37bc3fd3e05d

Subject:

JAB1, extracellular matrix deposition, inflammation, high glucose, glomerular mesangial cells, and ANGPTL2

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Diabetic or hyperglycaemic conditions stimulate the inflammatory response, excessive accumulation of extracellular matrix, and result in glomerulosclerosis, a scarring process of diabetic nephropathy. c-Jun activation domain-binding protein 1 (JAB1) functions as a regulator of pathways involved in cellular apoptosis and proliferation. The role of JAB1 in diabetic nephropathy was investigated in this study. Firstly, glomerular mesangial cells (GMCs) were treated with high glucose, and high glucose conditions induced up-regulation of JAB1 in the GMCs. Moreover, IL-6, TNF-α, MCP-1, and IL-1β were also elevated in high glucose-induced GMCs. Secondly, silencing of JAB1 reduced the levels of IL-6, TNF-α, MCP-1, and IL-1β in high glucose-induced GMCs. In addition, silencing of JAB1 attenuated the high glucose-induced decrease of superoxide dismutase (SOD) and the increase of reactive oxygen species (ROS) and malondialdehyde (MDA). The increased TGF-β1, collagen I, collagen IV, and fibronectin levels in high glucose-induced GMCs were restored by knockdown of JAB1. Thirdly, angiopoietin-like protein 2 (ANGPTL2) expression was reduced by JAB1. Over-expression of ANGPTL2 weakened the JAB1 silence-induced decrease of IL-6, TNF-α, MCP-1, IL-1β, TGF-β1, collagen I, collagen IV, and fibronectin. In conclusion, silencing of JAB1 reduced extracellular matrix deposition and suppressed inflammation in high glucose-induced GMCs through down-regulation of ANGPTL2.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

191-198

Source:

Folia biologica | 2021 Volume:67 | Number:5-6

Harvested from:

CDK

Metadata only:

false