The effect of chronic doxorubicin treatment on mitogen-activated protein kinases and heat stress proteins in rat hearts

Title:

The effect of chronic doxorubicin treatment on mitogen-activated protein kinases and heat stress proteins in rat hearts

Creator:

Šimončíková, P., Ravingerová, T., and Miroslav Barančík

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3d878a7c-706d-43b3-80b1-e9610883370c
uuid:3d878a7c-706d-43b3-80b1-e9610883370c
issn:0862-8408

Subject:

Experimentální medicína, kardiovaskulární fyziologie, proteinové kinázy, tepelný stres, srdce, cardiovascular physiology, protein kinases, heat stress, heart, doxorubicin, mitogen-activated protein kinase (MAPK), heat stress proteins, 14, and 616-092

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The study has been designed to characterize protein systems involved in the responses of rat hearts to chronic doxorubicin (DOX) treatment. We investigated the influence of DOX on cardiac function, mitogen-activated protein kinases (MAPKs) and heat stress proteins (HSPs). Doxorubicin was administered to rats by intraperitoneal injections over a period of 6 weeks. In control and DOX-treated hearts exposed to 20 min global ischemia and 40 min reperfusion the recovery of contractile function after ischemia/reperfusion (I/R) was determined. The levels and phosphorylation state of proteins in tissue samples were analyzed using specific antibodies. We found an activation of extracellular signal-regulated kinases (ERKs) in rat hearts exposed to DOX treatment and better recovery of contractile function after I/R. Analysis of HSPs showed that DOX induced up-regulation of the levels of HSP60 and down-regulation of HSP70 levels. The levels and/or specific phosphorylation of other studied proteins (p38-MAPK, HSP27, HSP90) were not in fluenced by DOX. The results point to the possible role of ERKs and some HSPs in mechanisms underlying the response of rat hearts to chronic DOX treatment., P. Šimončíková, T. Ravingerová, M. Barančík., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:Suppl 2

Harvested from:

CDK

Metadata only:

false