Effect of C677T Methylenetetrahydrofolate Reductase Gene Polymorphism on Plasma Homocysteine Levels in Ethnic Groups

Title:

Effect of C677T Methylenetetrahydrofolate Reductase Gene Polymorphism on Plasma Homocysteine Levels in Ethnic Groups

Creator:

Gašparovič, J., Rašlová, K., Bašistová, Z., Zacharová, M., Wsólová, L., Avdičová, M., Blažíček, P., Lietava, J., and Siváková, D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3fc51183-fb4c-436a-850f-44e94444cf12
uuid:3fc51183-fb4c-436a-850f-44e94444cf12

Subject:

C677T methylenetetrahydrofolate reductase polymorphism, Homocysteine, Slovak-Romanies, Slovak-Caucasians, and Ethnic groups

Type:

article, model:article, and TEXT

Description:

The objective of this study was to examine plasma homocysteine levels and C677T methylenetetrahydrofolate reductase (MTHFR) gene polymorphism in two ethnic groups from Slovakia. The samples consisted of general Slovak-Romany population (68 men and 81 women) from Southwestern Slovakia and the Slovak-Caucasians (174 men and 177 women) who participated in the CINDI project. The homocysteine levels were examined by HPLC, the analysis of MTHFR genotypes was done by PCR. The Slovak-Romany men (12.0±5.6 (S.D.) μmol/l) and women (9.2±2.6 μmol/l) have significantly lower plasma homocysteine levels (p<0.024 and p<0.00001) when compared to Caucasians (13.3±5.1 μmol/l in men and 11.3±4.3 μmol/l in women). The genetic equilibrium is assumed for the gene frequencies of the MTHFR polymorphism in both samples. The distribution of MTHFR genotypes did not differ between the two populations (TT 13 vs. 10.6 %; CT 46.6 vs. 41.7 %; CC 40.4 vs. 47.7%, zeta2 = 2.315, df=2, ns). The effect of MTHFR genotypes on homocysteine levels was not confirmed in the Slovak-Romanies and TT homozygosity significantly increased plasma homocysteine levels only in Slovak-Caucasians (11.5±4.4 mmol/l, ns; vs. 14.8±4.8 mmol/l, p<0.002, respectively). To our knowledge, this is the first epidemiological study in the Romany population examining distribution of the MTHFR genotypes and their effect on homocysteine levels. Further studies are needed to establish the variety of cardiovascular risk factors among Romanies in order to evaluate the significance of particular factors.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:2

Harvested from:

CDK

Metadata only:

false