Regulation of transepithelial ion transport in the rat late distal colon by the sympathetic nervous system

Title:

Regulation of transepithelial ion transport in the rat late distal colon by the sympathetic nervous system

Creator:

Zhang, X., Li, Y., Duan, Z., and Zhu, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:405c3a2e-02f7-46c7-a24a-a36a9c5cd567
uuid:405c3a2e-02f7-46c7-a24a-a36a9c5cd567
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, sympatický nervový systém, sympathetic nervous system, colorectum, ion transport, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The colorectum (late distal colon) is innervated by the sympathetic nervous system, and many colorectal diseases are related to disorders of the sympathetic nervous system. The sympathetic regulation of colorectal ion transport is rarely reported. The present study aims to investigate the effect of norepinephrine (NE) in the normal and catecholamine-depleted condition to clarify the regulation of the sympathetic adrenergic system in ion transport in the rat colorectum. NE-induced ion transport in the rats colorectum was measured by short-circuit current (Isc) recording; the expression of β-adrenoceptors and NE transporter (NET) were quantified by real-time PCR, and western blotting. When the endogenous catecholamine was depleted by reserpine, the baseline Isc in the colorectum was increased significantly comparing to controls. NE evoked downward ∆Isc in colorectum of treated rats was 1.8-fold of controls. The expression of β 2 -adrenoceptor protein in the colorectal mucosa was greater than the control, though the mRNA level was reduced. Ho wever, NET expression was significantly lower in catecholamine-depleted rats compared to the controls. In conclusion, the sympathetic nervous system plays an important role in regulating basal ion transport in the colorectum. Disorders of sympathe tic neurotransmitters result in abnormal ion transport, β-adrenoceptor and NET are involved in the process., X. Zhang, Y. Li, X. Zhang, Z. Duan, J. Zhu., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:1

Harvested from:

CDK

Metadata only:

false