The effect of antigen atimulation on splenic transplants

Title:

The effect of antigen atimulation on splenic transplants

Creator:

Bergmann, E. S., Leitner, W., Brtko, J., Boeckl, O., and Thalhamer, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:40b7fb90-2d47-4540-8d3e-391d0d4e5759
uuid:40b7fb90-2d47-4540-8d3e-391d0d4e5759

Subject:

splenic autotransplantation, immunohistology, flow-cytometry, antigen stimulation, and proliferation

Type:

article, model:article, and TEXT

Description:

The present paper deals with the regeneration of splenic tissue after autologous transplantation. Control and transplanted rats (60 days after operation (106 cells per injection). The effect of a primary response was studied by a single injection, long-lasting bacteraemia was imitated by 5 injections in weekly intervals. Spleens and transplants were investigated by flow-cytometry and immunohistochemistry. Additionally, the proliferation activity and the specific antibody production against Escherichia coli proteins were tested. Flow-cytometric analysis showed altered behaviour of T-helper cells and B-cells in transplants following a primary response, whereas in the multiple injection group a difference between the splenic and transplant response was restricted to macrophages and MHC II+ cells. The results of the morphometric analysis revealed that the cellular composition of unstimulated transplants was very similar to that of the spleen with some subtle alterations. Only the marginal zone showed more striking differences concerning the homing of several cell classes. Under stimulatory conditions, these subtle alterations became more drastic so that CD5 + cells, B-cells and macrophages responded in an abnormal manner in both groups. The analysis of thymidine kinase disclosed decreased activity in the spleen after weekly antigen stimulation. The stimulation index of all transplant groups was significantly lower than that of the spleen. The specific antibody (IgG) production after a single immunization was highest in the transplant group. All groups responded after the multiple challenge. In conclusion, the results demonstrate that splenic transplants differs in several, but subtle aspects from normal splenic tissue. The main reason for most of these alterations may be a slightly misguided recirculation and/or homing of cells.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1997 Volume:46 | Number:1

Harvested from:

CDK

Metadata only:

false