Gluconeogenesis during hypoxia in vascular smooth muscle studied by 13C-NMR

Title:

Gluconeogenesis during hypoxia in vascular smooth muscle studied by 13C-NMR

Creator:

Hardin, Christopher D. and Roberts, Tina M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:414b5cbd-2d44-43b5-be58-0cd9f532425f
uuid:414b5cbd-2d44-43b5-be58-0cd9f532425f

Subject:

hog carotid artery, glucose, and glycolysis

Type:

article, model:article, and TEXT

Description:

We investigated whether hypoxia altered the utilization of fructose-1,6-bisphosphate as a gluconeogenic or glycolytic intermediate in superfused media from hog carotid artery. Using 13C-NMR, we found that although 3-13C-iactate production from l-13C-glucose increased compared to that under well-oxygenated conditions, the conversion of exogenously applied l,6-13C-fructose-l,6-bisphosphate to glucose (gluconeogenesis) or to 3-13C-lactate was not significantly affected by hypoxia. Since hypoxia alters the rate of glucose conversion to lactate but not the conversion of fructose-1,6-bisphosphate to glucose, we conclude that glycolysis and glycogenolysis may continue to be compartmentalized during hypoxia and that a high rate of gluconeogenesis can occur even during hypoxia.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1995 Volume:44 | Number:4

Harvested from:

CDK

Metadata only:

false