Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera

Title:

Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera

Creator:

Xu, Peng Jun, Li, Tong, Xiao, Jin Hua, Murphy, Robert W., and Da Wei, Huang

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:416dcd0b-e3d6-4f6c-b5d6-97ce577abba7
uuid:416dcd0b-e3d6-4f6c-b5d6-97ce577abba7

Subject:

Zoologie, entomologie, universal primer, Lepidoptera, coding sequence, untranslated region, HSP90, RACE, 2, and 59

Type:

article, články, model:article, and TEXT

Description:

Using sequence alignment, a conserved domain in the 3' untranslated region (UTR) of the cytoplasmic heat shock protein 90 (HSP90) of Lepidoptera was found. This region is highly variable in other insect groups. Furthermore, universal primers were designed to amplify the complete coding sequence (CDS) of HSP90 from total genomic DNA in Lepidoptera, avoiding the commonly used reverse transcription-polymerase chain reaction (RT-PCR) and 3', 5'-rapid amplification of cDNA ends (RACE) methods based on cDNA. These primers amplified a fragment of about 2.25 kb in the 11 species tested, which represent seven different families of Lepidoptera, including moths and butterflies. The results suggest that the conserved domain of 3'UTR is universal in Lepidoptera and these primers successfully amplify the complete CDS of cytoplasmic HSP90 from genomic DNA. and Peng Jun XU, Tong LI, Jin Hua XIAO, Robert W. MURPHY, Huang DA WEI.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2011 Volume:108 | Number:1

Harvested from:

CDK

Metadata only:

false