Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment

Title:

Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment

Creator:

Jana Juhásová, Štefan Juhás, Jiří Klíma, Ján Strnádel, Holubová, M., and Jan Motlík

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:42946efe-987f-4acb-b949-37851356566c
uuid:42946efe-987f-4acb-b949-37851356566c

Subject:

Fyziologie člověka a srovnávací fyziologie, diferenciace buněk, popraviště, cell differentiation, scaffolds, mesenchymal stem cells, miniature pig, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteog enic differentiation of miniature pig MSCs and markers of this differentiation in vitro . Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma- alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. The weak initial osteonectin signal, culminating on 7th or 14th day of differentiation, depends on collagen I and vitronectin coating in 2D system. The highest activity of ALP was detected on 21th day of osteogenic differentiation. The PC scaffolds provided better conditions for osteogenic differentiation of MSCs than PAC scaffolds in vitro . We also observed expected effects of collagen I and vitronectin on the acceleration of osteogenic differentiation of miniature pig MSC. Our results indicate similar ability of miniature pig MSCs osteogenic differentiation in 2D and 3D environment, but the expression of osteogenic marker s in scaffolds and ECM coated monolayers started earlier than in the monolayers without ECM., J. Juhásová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:3

Harvested from:

CDK

Metadata only:

false