Biophysical investigation on left ventricular myocytes in rats with experimentally induced diabetes

Title:

Biophysical investigation on left ventricular myocytes in rats with experimentally induced diabetes

Creator:

Waczulíková, I., Cagalinec, M., Oľga Uličná, Peter Slezák, and Atila Ziegelhöffer

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:434c4727-76ec-42e6-9680-eba197142590
uuid:434c4727-76ec-42e6-9680-eba197142590

Subject:

Fyziologie člověka a srovnávací fyziologie, membrány, mitochondrie, fluorescenční spektroskopie, fluorescenční mikroskopie, membranes, mitochondrias, fluorescent spectroscopy, fluorescence microscopy, left ventricular myocytes, biophysical properties, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Diabetes is a recognized risk factor of heart disease. The abnormalities related to a decreased heart performance probably arise at cellular and molecular levels already in the asymptomatic phase of diabetes. However, the early alterations initiating a sequence of events that culminates in the clinical signs have not been fully elucidated yet. This review deals with some biophysical methods applied to investigation of left ventricular myocytes in rats with streptozotocin diabetes, as well as our most important findings concerning diabetes-induced cell changes which cannot be captured by other techniques. The observed decrease in sarcolemmal membrane fluidity is causatively associated with increased glycation and glycoxidation. On the other hand, an increase in the mitochondrial membrane fluidity may be attributed to augmented energy transduction through the membranes. We reported for the first time concurrent measurements of membrane potential and dynamics, and respiratory chain activities in rat heart mitochondria, as well as calcium transients in the myocytes from diabetic hearts together with the assessed quantitative relationships among these variables. We were able to detect some significant alterations that may underlie myocyte dysfunction and subsequent remodeling of the heart. We suppose that not all these changes reflect mechanisms leading to pathology; some may represent adaptive and compensatory responses to diabetes., I. Waczulíková ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false