RNA concentration and content in the nucleoli and cytoplasmic rim in differentiating lymphocytes of patients suffering from b chronic lymphocytic leukaemia - a cytochemical note

Title:

RNA concentration and content in the nucleoli and cytoplasmic rim in differentiating lymphocytes of patients suffering from b chronic lymphocytic leukaemia - a cytochemical note

Creator:

Smetana, Karel, Mikulenková, D., Karban, J., and Trněný, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:45565850-8e25-4808-aa4c-1488929fe96f
uuid:45565850-8e25-4808-aa4c-1488929fe96f

Subject:

RNA, leukemic lymphocytes, and cytochemistry

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

RNA optical density (concentration) measurements at the single cell level indicated that differentiation of lymphocytes is accompanied by a slightly decreased nucleolar RNA concentration in contrast to the cytoplasmic rim around the nucleus. On the other hand, the nucleolar size was markedly reduced and the cytoplasmic rim surrounding the nucleus was reduced only weakly. Concerning the calculated rough estimate of the RNA content, the differentiation induced its larger decrease in the nucleoli than in the cytoplasmic rim. These observations indicated that the nucleolar RNA concentration and RNA content together with the nucleolar morphology are more sensitive markers of the differentiation process than the RNA concentration and content in the cytoplasm. Thus, the nucleolar RNA transfer to the cytoplasm in advanced differentiation steps might still be going on regardless of the decreasing or inhibited nucleolar biosynthetic activity. In addition, the presence of ring-shaped nucleoli and micronucleoli characteristic of mature and terminal lymphocytes in some lymphocytic less differentiated steps, i.e., lymphoblasts and prolymphocytes, might indicate the premature differentiation state of such cells.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

40-44

Source:

Folia biologica | 2022 Volume:68 | Number:1

Harvested from:

CDK

Metadata only:

false