Physiological role of dendrotoxin-sensitive K+ channels in the rat cerebellar Purkinje neurons

Title:

Physiological role of dendrotoxin-sensitive K+ channels in the rat cerebellar Purkinje neurons

Creator:

Haghdoust, H., Janahmadi, M., and Behzadi, G.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:456444fc-c38b-43a6-b328-e699c30e15c0
uuid:456444fc-c38b-43a6-b328-e699c30e15c0
issn:0862-8408

Subject:

Obecná zoologie, fyziologie živočichů, draslík, neurony, animal physiology, potassium, neurons, Purkinje neurons, firing behaviour, α-dendrotoxin, 2, and 591

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

To understand the contribution of potassium (K+) channels, particularly α-dendrotoxin (D-type)-sensitive K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits), to the generation of neuronal spike output we must have detailed information of the functional role of these channels in the neuronal membrane. Conventional intracellular recording methods in current clamp mode were used to identify the role of α-dendrotoxin (α-DTX)-sensitive K+ channel currents in shaping the spike output and modulation of neuronal properties of cerebellar Purkinje neurons (PCs) in slices. Addition of α-DTX revealed that D-type K+ channels play an important role in the shaping of Purkinje neuronal firing behavior. Repetitive firing capability of PCs was increased following exposure to artificial cerebrospinal fluid (aCSF) containing α-DTX, so that in response to the injection of 0.6 nA depolarizing current pulse of 600 ms, the number of action potentials insignificantly increased from 15 in the presence of 4-AP to 29 action potentials per second after application of DTX following pretreatment with 4-AP. These results indicate that D-type K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits) may contribute to the spike frequency adaptation in PCs. Our findings suggest that the activation of voltage-dependent K+ channels (D and A types) markedly affect the firing pattern of PCs., H. Haghdoust, M. Janahmadi, G. Behzadi., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:6

Harvested from:

CDK

Metadata only:

false