Effect on exercise on augmented aortic vasoconstriction in the db/db mouse model of type-II diabetes

Title:

Effect on exercise on augmented aortic vasoconstriction in the db/db mouse model of type-II diabetes

Creator:

Khazaei, M., Moien-Afshari, F., Kieffer, T. J., and Laher, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:45d09073-fb62-4d93-b1bd-3d57579620d3
uuid:45d09073-fb62-4d93-b1bd-3d57579620d3
issn:0862-8408

Subject:

Experimentální medicína, fyziologie, diabetologie, tělesná cvičení, physiology, diabetology, exercises, vasoconstriction, diabetes, 14, and 616-092

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

We evaluated the effects of exercise on the vascular constrictor responses to α-adrenergic stimulation in the db/db mice. Twenty male db/db and their age-matched wild-type (WT) mice were exercised (1 hour/day, five days a week). Mice were anesthetized 7 weeks later, thoracic aortae were mounted in wire myograph and constrictor responses to phenylephrine (PE, 1 nM-10 μM) were obtained. Citrate synthase activity measured in the thigh adductor muscle was significantly increased in db/db mice that were exercise trained. Maximal force generated by PE was markedly greater in db/db aortae and exercise did not attenuate this augmented contractile response. Vessels were incubated with inhibitors of nitric oxide synthase (L-NAME, 200 μM), endothelin receptors (bosentan, 10 μM), protein kinase C (PKC) (calphostin C, 5 μM), cyclooxygenase (indomethacin, 10 μM) or Rho-kinase (Y-27632, 0.1 μM). Only calphostin-C normalized the augmented PE-induced constriction in db/db and db/db- exercised mice to that observed in WT (p<0.05). Cumulative additions of indolactam, a PKC activator, induced significantly greater constrictor responses in aortic rings of db/db mice compared to WT and exercise did not affect this response. Our data suggest that the augmented vasoconstriction observed in the aorta of db/db mice is likely due to increased PKC activity and that exercise do not ameliorate this increased PKC-mediated vasoconstriction., M. Khazaei, F. Moien-Afshari, T. J. Kieffer, I. Laher., and Obsahuje bibliografii a bibliiografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:6

Harvested from:

CDK

Metadata only:

false