Molecular evidence suggests the occurrence of Entamoeba moshkovskii in pigs with zoonotic potential from eastern India

Title:

Molecular evidence suggests the occurrence of Entamoeba moshkovskii in pigs with zoonotic potential from eastern India

Creator:

Sardar, Sanjib K., Das, Koushik, Maruf, Maimoon, Haldar, Tapas, Saito-Nakano, Yumiko, Kobayashi, Seiki, Dutta, Shanta, and Ganguly, Sandipan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:48c2e5bd-9019-4425-b126-7d65440e7972
uuid:48c2e5bd-9019-4425-b126-7d65440e7972
doi:10.14411/fp.2022.012

Subject:

PCR, RFLP, host, natural reservoir, intestinal protists, and diarrhea

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Entamoeba moshkovskii Tshalaia, 1941 is prevalent in developing countries and it is considered to be primarily a free-living amoeba, which is morphologically indistinguishable, but biochemically and genetically different from the human infecting, pathogenic Entamoeba histolytica Schaudinn, 1903. The pathogenic potential of this organism is still under discussion. Entamoeba moshkovskii in human stool samples has been reported in different countries such as the United States, Italy, Australia, Iran, Turkey, Bangladesh, India (Pondicherry), Indonesia, Colombia, Malaysia, Tunisia, Tanzania and Brazil, but no data are available about the occurrence of E. moshkovskii in farm animals. This study provides data on the occurrence of E. moshkovskii in pigs in a total of 294 fresh faecal samples collected from five different regions in Kolkata, West Bengal, India. Stool samples were tested by nested PCR using primers targeting SSU rDNA of E. moshkovskii. The amplified PCR products were further confirmed by RFLP technique. Purified nested PCR products were also sequenced and identified via BLAST program run on the NCBI website to confirm species along with their genetic characteristics of the E. moshkovskii isolates. Overall 5.4 % samples were identified as E. moshkovskii positive. Results of this study demonstrate that swine can host E. moshkovskii and should be considered as a potential natural reservoir for E. moshkovskii. However, the occurrence of E. moshkovskii infection in pigs was not statistically associated with their faecal consistency, sex and developmental stage.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

1-6

Source:

Folia parasitologica | 2022 Volume:69 | Number:1

Harvested from:

CDK

Metadata only:

false