Allelic variants of cytochrome P450 monooxygenases: Constitutive and insecticide-mediated expression in a Malaysian strain of the dengue vector, Aedes aegypti (Diptera: Culicidae)

Title:

Allelic variants of cytochrome P450 monooxygenases: Constitutive and insecticide-mediated expression in a Malaysian strain of the dengue vector, Aedes aegypti (Diptera: Culicidae)

Creator:

El-Garj, Fatma M. A, Wajidi, Mustafa F. F., and Avicor, Silas W.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4b68d732-8840-4096-8fcb-124ed745232d
uuid:4b68d732-8840-4096-8fcb-124ed745232d
issn:1210-5759
doi:10.14411/eje.2016.067

Subject:

Zoologie, Diptera, Culicidae, Aedes aegypti, cytochrome P450s, structure prediction, mRNA expression, insecticide, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

Cytochrome P450s (P450s) involved in insecticide resistance reduce the efficacy of insecticide-based vector control by rendering vector control ineffective. They are recorded in many species of vectors and have various constitutive and insecticide induction profiles. In this study, the isolation and prediction of the structure of a P450 from a strain of Aedes aegypti originating from Malaysia is reported. Quantitative mRNA expression of this gene and a previously reported P450, CYP4H28v2, in the developmental stages of the mosquito after exposure to sub-lethal concentrations of insecticides is also reported. The isolated P450, CYP4H31v2, is an allelic variant of CYP4H31 and contains several conserved motifs of P450s. The secondary structure of the protein is mostly made up of alpha helices and random coils. The tertiary structure was generated using homology modeling and was of good quality based on structure validation using protein structure assessment tools. CYP4H28v2 and CYP4H31v2 were differentially expressed in the developmental stages of the vector, with a significantly increased expression in adult males. The genes were significantly over-expressed in larvae exposed to deltamethrin and permethrin for 6 h. In the DDT-treated larvae, only CYP4H31v2 was significantly over-expressed after a 6 h exposure. Under-expression of the genes was predominant in larvae treated with the organophosphates malathion and temephos. Though the functions of these P450s are unknown, their response to induction by exposure to insecticides indicates the likely involvement of these genes in insecticide tolerance. and Fatma M. A. El-Garj, Mustafa F.F. Wajidi, Silas W. Avicor.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2016 Volume:113

Harvested from:

CDK

Metadata only:

false