Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity

Title:

Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity

Creator:

Franck, Thierry, Kohnen, S., Grulke, S., Patrick Neven, Goutman, Y., Peters, F., Pirotte, B., Deby-Dupont, G., and Serteyn, D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4c2150d8-680d-4211-80c6-abe95e423cd1
uuid:4c2150d8-680d-4211-80c6-abe95e423cd1
issn:0862-8408

Subject:

fyziologie, lékařská chemie, koně, physiology, medical chemistry, horses, curcuminoids, stimulated equine neutrophils, and myeloperoxidase activity

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In the horse, the inflammation response to various pathologies (intestinal strangulations, laminitis, etc.) involves an excessive stimulation of the polymorphonuclear neutrophils releasing reactive oxygen species (ROS) and myeloperoxidase (MPO). The aim of the present work was to study the effect of natural polyphenols, curcumin oids and tetrahydrocurcuminoids (THC) on isolated stimulated equine neutrophils and on the activity of purified MPO. The ROS production and the release of MPO by activated neutrophils were measured by chemiluminescence and ELISA techniques, respectively. The activity of purified MPO was measured by studying its nitration, chlorination or oxidation capacity and by using an original method called SIEFED allowing the study of drug interaction with the enzyme without interferences of the medium. Curcuminoids and THC had dose-dependent inhibitory effects on ROS production and MPO release by activated neutrophils and on purified MPO activity. We suggest that the higher efficacy of curcuminoids versus THC could be explained, at least partially, by its chemical structure: the conjugated double bounds and the plane structure of curcuminoids made easier the neutralization of the radical species generated by activated neutrophils and the interaction of the drug with the active site of MPO. These inhibitory effects of curcuminoids on the oxidant activity of equine neutrophils and on MPO activity open therapeutic perspectives in equine pathologies with excessive inflammatory reactions., T. Franck, S. Kohnen, S. Grulke, P. Neven, Y. Goutman, F. Peters, B. Pirotte, G. Deby-Dupont, D. Serteyn., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:4

Harvested from:

CDK

Metadata only:

false