Decreased hemojuvelin protein levels in mask mice lacking matriptase-2-dependent proteolytic activity

Title:

Decreased hemojuvelin protein levels in mask mice lacking matriptase-2-dependent proteolytic activity

Creator:

Frýdlová, J., Fujikura, Y., Martin Vokurka, Emanuel Nečas, and Jan Krijt

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:50012346-c40c-42a4-a218-372f90135339
uuid:50012346-c40c-42a4-a218-372f90135339

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, hepcidin, Id1, tmprss6, Hfe2, transferrin receptor 2, neogenin, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Matriptase-2, a membrane protein encoded by the Tmprss6 gene, is a negative regulator of hepcidin expression. Although matriptase-2 has been proposed to cleave membrane hemojuvelin, we have recently found decreased hemojuvelin protein levels in Tmprss6 -/- mice. The purpose of this study was to confirm this observation by determining hemojuvelin protein levels in another strain of mice with disrupted Tmprss6 gene, and to determine the effect of matriptase-2 deficiency on the expression of other membrane proteins participating in the bone morphogenetic protein signal transduction. Mask mice, which lack the proteolytic domain of matriptase-2, displayed decreased liver hemojuvelin protein content, while Id1 mRNA level, an indicator of hemojuvelin-dependent signal transduction, was increased. Protein levels of bone morphogenetic protein receptors Alk3 and Acvr2a were unchanged, and tran sferrin receptor 2 and neogenin protein levels were slightly decreased. The results confirm that the loss of matriptase-2 increases bone morphogenetic protein- dependent signaling, while pa radoxically decreasing liver hemojuvelin protein content. The regulation of transferrin receptor 2 protein levels by transferrin saturation was not affected in mask mice. How the loss of matriptase-2 proteolytic activity leads to decreased hemojuvelin protein levels is at present unclear., J. Frýdlová, ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2013 Volume:62 | Number:4

Harvested from:

CDK

Metadata only:

false