Caspase 3 Activation in the Primary Enamel Knot of Developing Molar Tooth

Title:

Caspase 3 Activation in the Primary Enamel Knot of Developing Molar Tooth

Creator:

Matalová, E., Kovářů, F., and Míšek, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5508a71b-064b-4803-9921-bbd1eae78b67
uuid:5508a71b-064b-4803-9921-bbd1eae78b67

Subject:

Apoptosis, Capase 3, Primary enamel knot, and Tooth development

Type:

article, model:article, and TEXT

Description:

Mammalian teeth develop during embryogenesis as epithelio-mesenchymal organs. The primary enamel knot is considered as a signaling center in tooth morphogenesis. After tooth bell formation, this epithelial structure undergoes apoptosis. Activation of caspase 3 represents a crucial step in the intracellular death machinery. Procaspase 3 and caspase 3 molecules were localized in the primary enamel knot of the field vole using immunohistochemistry. Different fixation procedures in cryopreserved and paraffin-embedded tissues and detection systems based on peroxidase and alkaline phosphatase mediated color reactions were applied. Apoptosis was detected using morphological criteria and the TUNEL assay. Procaspase 3 was found in both the epithelial and mesenchymal part of the tooth germ. Active caspase 3 was localized particularly in the primary enamel knot, its distribution correlated with dental apoptosis and showed a similar pattern in the field vole as in the mouse.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:2

Harvested from:

CDK

Metadata only:

false