Nanofat 2.0: experimental evidence for a fat grafting rich in mesenchymal stem cells

Title:

Nanofat 2.0: experimental evidence for a fat grafting rich in mesenchymal stem cells

Creator:

Lo Furno, D., Tamburino, S., Mannino, G., Gili, E., Lombardo, G., Tarico, M. S., Vancheri, C., Giuffrida, R., and Perrotta, R. E.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:56958925-6142-4f26-b77d-185b247a3793
uuid:56958925-6142-4f26-b77d-185b247a3793
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, proliferace buněk, regenerační medicína, cell proliferation, regenerative medicine, fat grafting, adipose stem cells, 14, and 612

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Different strategies have been developed in the last decade to obtain fat grafts as rich as possible of mesenchymal stem cells, so exploiting their regenerative potential. Recently, a new kind of fat grafting, called "nanofat", has been obtained after several steps of fat emulsification and filtration. The final liquid suspension, virtually devoid of mature adipocytes, would improve tissue repair because of the presence of adipose mesenchymal stem cells (ASCs). However, since it is probable that many ASCs may be lost in the numerous phases of this procedure, we describe here a novel version of fat grafting, which we call "nanofat 2.0", likely richer in ASCs, obtained avoiding the final phases of the nanofat protocol. The viability, the density and proliferation rate of ASCs in nanofat 2.0 sample were compared with samples of nanofat and simple lipoaspirate. Although the density of ASCs was initially higher in lipoaspirate sample, the higher proliferation rate of cells in nanofat 2.0 virtually filled the gap within 8 days. By contrast, the density of ASCs in nanofat sample was the poorest at any time. Results show that nanofat 2.0 emulsion is considerably rich in stem cells, featuring a marked proliferation capability., D. Lo Furno, S. Tamburino, G. Mannino, E. Gili, G. Lombardo, M. S. Tarico, C. Vancheri, R. Giuffrida, R. E. Perrotta., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:4

Harvested from:

CDK

Metadata only:

false