Connexin 50 mutation lowers blood pressure in spontaneously hypertensive rat

Title:

Connexin 50 mutation lowers blood pressure in spontaneously hypertensive rat

Creator:

Ondřej Šeda, František Liška, Michal Pravenec, Zdenka Vernerová, Ludmila Kazdová, Drahomíra Křenová, Václav Zídek, Lucie Šedová, Michaela Krupková, and Vladimír Křen

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:57546dd9-ca5a-403a-ab67-944dce5133d9
uuid:57546dd9-ca5a-403a-ab67-944dce5133d9
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hypertenze, inzulinová rezistence, hypertension, insulin resistance, connexin, transcriptome, animal models, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

We assessed the effect of the previously uncovered gap junctio n protein alpha 8 (Gja8) mutation present in spontaneously hypertensive rat - dominant cataract (SHR - Dca ) strain on blood pressure, metabolic profile, and heart and renal transcriptomes. Adult, standard chow-fed male rats of SHR and SHR - Dca strains were used. We found a significant, consistent 10-15 mmHg decrease in both systolic and diastolic blood pressures in SHR - Dca compared with SHR (P<0.01 and P<0.05 , respectively; repeated measures analysis of variance (ANOVA)). With immunohistochemistry, we were able to localize Gja8 in heart, kidney, aorta, liver, and lungs, mostly in endothelium; with no differences in expression between strains. SHR - Dca rats showed decreased body weight, high-density lipoprotein cholesterol concentrations and basa l insulin sensitivity in muscle. There were 21 transc ripts common to the sets of 303 transcripts in kidney and 487 in heart showing >1.2-fold difference in expression between SHR and SHR - Dca. Tumor necrosis factor was the most significant upstream regulato r and glial cell-derived neurotrophic factor family ligand-receptor interactions was the common enriched and downregulated canonical pathway both in heart and kidney of SHR - Dca. The connexin 50 mutation L7Q lowers blood pressure in the SHR - Dca strain, decr eases high-density lipoprotein cholesterol, and leads to substantial transcriptome changes in heart and kidney., O. Šeda, F. Liška, M. Pravenec, Z. Vernerová, L. Kazdová, D. Křenová, V. Zídek, L. Šedová, M. Krupková, V. Křen., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:1

Harvested from:

CDK

Metadata only:

false