Actin and major sperm protein in spermatozoa of a nematode, Graphidium strigosum (Strongylida: Trichostrongylidae)

Title:

Actin and major sperm protein in spermatozoa of a nematode, Graphidium strigosum (Strongylida: Trichostrongylidae)

Creator:

Mansir, Aicha and Justine, Jean-Lou

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:57df2ec3-49d0-4c03-a067-1d93be46629b
uuid:57df2ec3-49d0-4c03-a067-1d93be46629b
urnnbn:urn:nbn:cz:aba007-000dqr

Subject:

actin, major-sperm-protein, cytoskeleton, spermatozoon, immunocytochemistry, and nematode

Type:

model:internalpart and TEXT

Format:

print

Description:

In most amoeboid cells, the main protein involved in motility is actin. Nematode sperm are an exception, and their amoeboid motility is based on major sperm protein (MSP). We have studied the localization of actin and MSP in spermatids and spermatozoa of Graphidium strigosum (Dujardin, 1845), a species which has elongate male germ cells in which organelles are easily identified. Electrophoreses of G. slrigosum sperm proteins indicate that the main protein band, about 15 kDa in molecular weight, is specifically recognized by an anti-MSP polyclonal antibody developed against MSP of Caenorhabditis elegans (Burke and Ward 1983). Actin is present in small quantities. Immunocytochemical observations reveal that actin and MSP have an identical localization in precise areas of the male germ cells. Spermatids are labelled as dots around a central unlabelled zone, and spermatozoa are labelled only at the level of the anterior cap. Observations in G. strigosum are similar to that previously obtained in Heligmosomoides polygyrus (Mansir and Justine 1996). Co-localization of actin and MSP in the anterior cap of the spermatozoon, the region associated with pseudopod production, does not demonstrate directly that actin is involved in amoeboid movements, but shows that the role of actin in the cytoskeleton of nematode sperm should be re-investigated.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

47-51 and 49-53

Source:

Folia parasitologica | 1999 Volume:46 | Number:1

Harvested from:

CDK

Metadata only:

false