Differential sensitivity of the brain ATP-dependent and GTP- dependent succinyl-CoA synthetase to vanadium Ions. Developmental aspects

Title:

Differential sensitivity of the brain ATP-dependent and GTP- dependent succinyl-CoA synthetase to vanadium Ions. Developmental aspects

Creator:

Křivánek, J. and Nováková, L.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5bab8a7d-e352-458c-9368-c5761e0f4a3f
uuid:5bab8a7d-e352-458c-9368-c5761e0f4a3f

Subject:

succinyl-CoA synthetase, vanadate, vanadyl, brain, and development

Type:

article, model:article, and TEXT

Description:

We have recently found that both vanadate and vanadyl inhibit ATP-dependent succinyl-CoA synthetase (A-SCS) solubilized from the rat brain mitochondria. Aim of the present study was to estimate a proportion of A-SCS to G-SCS in adult and 5-day-old rat brain and their susceptibility to vanadium ions. The G-SCS to A-SCS ratio of 5-day-old brains was by 196 % higher than that in adults. This is in accordance with previous observation that G-SCS is high in tissues metabolizing ketone bodies. Both G-SCS and A-SCS differ in their susceptibility towards vanadium ions. A-SCS of adult brain was more sensitive to vanadate (IC 50 1.6.10'5 mol.I"1) than was G-SCS (IC 50 6.2.10"5 molT1). On the contrary G-SCS was more sensitive to vanadyl (IC 50 3.5.10^ mol.I1) than was A-SCS (IC 50 9.0.10-4 mol.I1). Also autophosphorylation of G-SCS a-subunit was more resistant to vanadate than A-SCS. In contrast to the adult SCS forms, almost equal susceptibility of A-SCS and G-SCS to vanadyl and vanadate was observed in infant brains. The results suggest some structural (functional) differences between two SCS forms in adults and also between infant and adult G-SCS.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1992 Volume:41 | Number:5

Harvested from:

CDK

Metadata only:

false