Growth in length and weight, based on a combination of scale annulus interpretation and back-calculation using the Fraser-Lee model, was studied in male and female barbel, Barbus barbus, from a section of the River Jihlava sampled in 1999–2001. Results were compared with growth data obtained with similar methods in 1976, prior to construction and functioning of a hydropower scheme complex, and during the period of the scheme’s partial operation (1980–1984). Recent growth rate, under seemingly fully-stabilised environmental conditions and complete adaptation of the barbel population, showed the highest distinct sexual dimorphism in growth rate was also confirmed, with females growing faster than males, though to a lower extent than recorded both during previous periods and from several other localities. Further, upon comparison of back-calculated lengths for previous years of recently tagged-and-recaptured fish (1999–2001) with observed lengths directly measured at corresponding ages, no significant differences were overall found between the results obtained by either method in most age groups. Finally, the linear Fraser-Lee model proved a sufficiently accurate and practical method for back-calculating lengths for previous years of life also in barbel.