Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)

Title:

Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)

Creator:

Macours, Nathalie, Vandingenen, Anick, Gielens, Constant, Hens, Korneel, Baggerman, Geert, Schoofs, Liliane , and Huybrechts, Roger

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:606cf6cc-e4d6-4f60-95c6-1ce3a270b23d
uuid:606cf6cc-e4d6-4f60-95c6-1ce3a270b23d

Subject:

ACE inhibitors, cDNA cloning, insects, Locusta migratoria, reproduction, and testes

Type:

article, model:article, and TEXT

Description:

By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial amino-acid sequence homology with ACE from both vertebrate and invertebrate origin. The ACE identity of the purified enzyme was further confirmed by cDNA cloning of the Locusta ACE fragment, which, after in silico translation, revealed a mature protein of 623 amino acids with a large structural similarity to other known ACE proteins.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2003 Volume:100 | Number:4

Harvested from:

CDK

Metadata only:

false