Circulating cell-free DNA extraction from liquid biopsy for cancer research

Title:

Circulating cell-free DNA extraction from liquid biopsy for cancer research

Creator:

Pfeiferova, L., Safarikova, M., Ulrych, J., Krska, Z., Frankova, V., Zima, T., and Kalousova, Marta

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:62d9309a-a3ac-4561-adf7-b72c1ba0e36a
uuid:62d9309a-a3ac-4561-adf7-b72c1ba0e36a

Subject:

biomarker, cancer, cell-free DNA, circulating tumour DNA, liquid biopsy, oncology, and precision

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

As the number of cancer patients globally increases, a need for reliable biomarkers including circulating tumour DNA from liquid biopsy for diagnosis, prognosis and monitoring of the disease is rising. Currently, mainly tissue samples from biopsy are used, but there are certain limitations: firstly, it is an invasive technique, and secondly, in some cases it is almost impossible to obtain an acceptable tissue sample. This could be changed by using circulating cell-free DNA from liquid biopsy, which also gives the possibility of repeated examination. Here, we focus on the options of isolating circulating cell-free DNA from plasma samples using two isolation techniques: precision manual QIAamp Circulating Nucleic Acid Kit and automatic MagNA Pure Compact (MPC) using Nucleic Acid Isolation Kit I. Manual extraction gave significantly better yields of circulating tumour DNA (P < 0.05). This DNA also had less contaminants (organic compounds or proteins). DNA obtained by both tested methods of isolation is suitable for subsequent molecular genetic methods.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

153-157

Source:

Folia biologica | 2022 Volume:68 | Number:4

Harvested from:

CDK

Metadata only:

false