The influence of monovalent cations on trimeric G protein Gi1α activity in HEK293 cells stably expressing DOR-Gi1α (Cys351-Ile351) fusion protein

Title:

The influence of monovalent cations on trimeric G protein Gi1α activity in HEK293 cells stably expressing DOR-Gi1α (Cys351-Ile351) fusion protein

Creator:

Vošahlíková, M. and Petr Svoboda

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:639dfe55-30a5-4b17-9485-fb62ef44b6db
uuid:639dfe55-30a5-4b17-9485-fb62ef44b6db

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, δ-opioid receptor (DOR), monovalent ions, Gi1α protein, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

The effect of monovalent cations on trimeric G protein Gi1α was measured at equimolar concentration of chloride anion in pertussis-toxin (PTX)-treated HEK293 cells stably expressing PTX- insensitive DOR-Gi1α (Cys351-Ile351) fusion protein by high-affinity [35S]GTPγS binding assay. The high basal level of binding was detected in absence of DOR ag onist and monovalent ions and this high level was inhibited with the order of: Na+>K+>Li+. The first significant inhibition was detected at 1 mM NaCl. The inhibition by monovalent ions was reversed by increasing concentrations of DOR agonist DADLE. The maximum DADLE response was also highest for sodium and decreased in the order of: Na+>K+≈Li+. Our data indicate i) an inherently high activity of trimeric G protein G i 1 α when expressed within DOR-Gi1α fusion protein and determined in the absence of monovalent cations, ii) preferential sensitivity of DOR-Gi1α to sodium as far as maximum of agonist response is involved., M. Vošahlíková, P. Svoboda., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:3

Harvested from:

CDK

Metadata only:

false