Tri-lineage differentiation of NTERA2 clone D1 cells towards neural, hepatic and osteogenic lineages in vitro

Title:

Tri-lineage differentiation of NTERA2 clone D1 cells towards neural, hepatic and osteogenic lineages in vitro

Creator:

Szeky, Balázs, Mayer, B., Gyongy, M., Hajdara, A., Barsi, S., Karpati, S., and Nemeth, K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6711e975-262e-4884-8084-ed0717e5211c
uuid:6711e975-262e-4884-8084-ed0717e5211c

Subject:

NTERA2 clone D1 cells, neurogenesis, hepatogenesis, osteogenesis, and tri-lineage differentiation

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Over the past decades, the in vitro use of pluripotent cell lines gained a crucial role in toxicology, preclinical drug testing and developmental biology. NTERA2 clone D1 cells were identified as pluripotent cells with high potential for neural differentiation. Although they are commonly used cellular sources in neuropharmacology and neurodevelopmental studies, their endodermal and mesodermal differentiation potential awaits further characterization. Here, we devised improved protocols for hepatogenic and osteogenic differentiation of NTERA2 clone D1 cells. Our in vitro differentiation assays showed significant up-regulation of multiple hepatogenic markers. We also observed robust mineralization and osteogenic marker expression of NTERA2 clone D1 cells upon in vitro osteogenic induction. These results suggest that NTERA2 clone D1 cells may be utilized as an in vitro model system to study various aspects of liver biology and osteogenesis. In addition, tri-lineage differentiation of NTERA2 clone D1 cells may serve as a simple experimental control system when validating pluripotency of other cell types.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

174-182

Source:

Folia biologica | 2021 Volume:67 | Number:5-6

Harvested from:

CDK

Metadata only:

false