Fluid shear stress-induced down-regulation of miR-146a-5p inhibits osteoblast apoptosis via targeting SMAD4

Title:

Fluid shear stress-induced down-regulation of miR-146a-5p inhibits osteoblast apoptosis via targeting SMAD4

Creator:

Liu, Xuening, Zhang, Kun, Wang, Lifu, Geng, Bin, Liu, Zhongcheng, Yi, Qiong, and Xia, Yayi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:67c8780d-bc66-4b48-8131-8f0193b4c835
uuid:67c8780d-bc66-4b48-8131-8f0193b4c835
doi:10.33549/physiolres.934922

Subject:

fluid shear stress, miR-146a-5p, SMAD4, and osteoblast apoptosis

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Fluid shear stress (FSS) plays an important role in osteoblast apoptosis. However, the role of miRNA in osteoblast apoptosis under FSS and possible molecular mechanisms remain unknown. Our aim of the study was to explore whether miR-146a-5p regulates osteoblast apoptosis under FSS and its molecular mechanisms. FSS could down-regulate the expression of miR146a-5p in MC3T3-E1 cells. We confirm that up-regulation of miR-146a-5p promotes osteoblasts apoptosis and downregulation of miR-146a-5p inhibits osteoblasts apoptosis. We further demonstrated that FSS inhibits osteoblast apoptosis by down-regulated miR-146a-5p. Dual-luciferase reporter assay validated that SMAD4 is a direct target gene of miR-146a-5p. In addition, mimic-146a-5p suppressed FSS-induced up-regulation of SMAD4 protein levels, which suggests that FSS elevated SMAD4 protein expression levels via regulation miR-146a-5p. Further investigations showed that SMAD4 could inhibit osteoblast apoptosis. We demonstrated that miR-146a-5p regulates osteoblast apoptosis via targeting SMAD4. Taken together, our present study showed that FSS-induced downregulation miR-146a-5p inhibits osteoblast apoptosis via target SMAD4. These findings may provide novel mechanisms for FSS to inhibit osteoblast apoptosis, and also may provide a potential therapeutic target for osteoporosis.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

835-848

Source:

Physiological research | 2022 Volume:71 | Number:6

Harvested from:

CDK

Metadata only:

false