Methodology for intravital mitotic chromosome preparation from regenerated tissue derived from the tail tips of tadpoles

Title:

Methodology for intravital mitotic chromosome preparation from regenerated tissue derived from the tail tips of tadpoles

Creator:

Pustovalova, Eleonora, Fedorova, Anna, and Shabanov, Dmytro

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:71374c7b-7ea3-4dd8-b1da-f904f4728c7b
uuid:71374c7b-7ea3-4dd8-b1da-f904f4728c7b
doi:10.25225/jvb.22010

Subject:

karyoanalysis, Pelophylax, polyploidy, and regeneration

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

We propose a modified and updated protocol to obtain mitotic chromosomes from the regenerated tissue of Pelophylax tadpole tail tips. Chromosomal preparations from regenerated tissue results in high-quality and clean slides suitable for further staining and study. Tadpoles remain alive, undergo minimum suffering, and can be grown to adulthood for further investigation. The method could be used for other groups of Anura and modified for other species with the ability to regenerate their tissues.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

1-7

Source:

Journal of Vertebrate Biology | 2022 Volume:71 | Number:1

Harvested from:

CDK

Metadata only:

false