MENS-associated increase of muscular protein content via modulation of caveolin-3 and TRIM72

Title:

MENS-associated increase of muscular protein content via modulation of caveolin-3 and TRIM72

Creator:

Ohno, Y., Egawa, T. , Yokoyama, S., Fujiya, H., Sugiura, T. , Ohira, Y. , Yoshioka, T. , and Goto, K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:723a8416-388d-4a1f-9716-12b359bbf650
uuid:723a8416-388d-4a1f-9716-12b359bbf650
doi:10.33549/physiolres.933992

Subject:

Skeletal muscle cells, Microcurrent electrical neuromuscular stimulation, Protein content, Caveolin-3, and Tripartite motifcontaining 72

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Microcurrent electrical neuromuscular stimulation (MENS) is known as an extracellular stimulus for the regeneration of injured skeletal muscle in sports medicine. However, the effects of MENS-associated increase in muscle protein content are not fully clarified. The purpose of this study was to investigate the effects of MENS on the muscular protein content, intracellular signals, and the expression level of caveolin-3 (Cav-3), tripartite motifcontaining 72 (TRIM72) and MM isoenzyme of creatine kinase (CK-MM) in skeletal muscle using cell culture system. C2C12 myotubes on the 7th day of differentiation phase were treated with MENS (intensity: 10-20 μA, frequency: 0.3 Hz, pulse width: 250 ms, stimulation time: 15-120 min). MENS-associated increase in the protein content of myotubes was observed, compared to the untreated control level. MENS upregulated the expression of Cav-3, TRIM72, and CK-MM in myotubes. A transient increase in phosphorylation level of Akt was also observed. However, MENS had no effect on the phosphorylation level of p42/44 extracellular signal-regulated kinase-1/2 and 5’AMP-activated protein kinase. MENS may increase muscle protein content accompanied with a transient activation of Akt and the upregulation of Cav-3 and TRIM72.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

265-273

Source:

Physiological research | 2019 Volume:68 | Number:2

Harvested from:

CDK

Metadata only:

false