Characterization of the capillary network in skeletal muscles from 3D data

Title:

Characterization of the capillary network in skeletal muscles from 3D data

Creator:

Ida Eržen, Jiří Janáček, and Lucie Kubínová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7391277c-dfa6-4df1-8661-c185c0179f62
uuid:7391277c-dfa6-4df1-8661-c185c0179f62

Subject:

Fyziologie člověka a srovnávací fyziologie, svaly, kapilarita, muscles, capillarity, capillaries, skeletal muscle, confocal microscopy, stereology, 3D analysis, 3D visualization, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

In this review we present immunohistochemical methods for visualization of capillaries and muscle fibres in thick muscle sections. Special attention is paid to the procedures that preserve good morphology. Applying confocal microscopy and virtual 3D stereological grids, or tracing of capillaries in virtual reality, length of capillaries within a muscle volume or length of capillaries adjacent to a muscle fibre per fibre length, fibre surface area or fibre volume can be evaluated by an unbiased approach. Moreover, 3D models of capillaries and muscle fibres can be produced. Comparison of the developed methods with counting capillary profiles from 2D sections is discussed and the reader is warned that counting capillary profiles from 2D sections can underestimate the capillary length by as much as 75 percent. Application of the described 3D methodology is illustrated by the anatomical remodelling of capillarity during acute denervation and early reinnervation in the ra t soleus and extensor digitorum longus muscles., I. Eržen, J. Janáček, L. Kubínová., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:1

Harvested from:

CDK

Metadata only:

false